Brucellosis is a zoonosis caused by Brucella, with an acute or chronic clinical infection. Clinical manifestations of brucellosis is various or atypical, which is easily misdiagnosed and miss-diagnosed. The case we have reported had an onset of fever and abdominal pain, associated with arthralgia, headache and rashes during the course, and was initially misdiagnosed as “acute cholangitis, associated with biliary pancreatitis”. According to epidemiology survey, radiological evidence, as well as blood culture and agglutination test showing Brucella positive, the diagnosis of brucellosis associated with splenic infarction and multiple organ involvement including lungs, skin and brain was made. After regular anti-brucellosis treatment, the patient’s symptoms were significantly improved. Brucellosis associated with splenic infarction is rare, which is possibly due to vasculitis resulting from Brucella infection.