With the wide use of antifungals in the clinic, there have been increasing reports of resistant strains of Aspergillus spp. to antifungals. The resistance of Aspergillus spp. has important impact on the diagnosis and treatment of invasive aspergillosis. Currently, the determination of antifungal resistance of pathogenic Aspergillus spp. relies on antifungal susceptibility testing and molecular detection. Recently, the resistance of Aspergillus spp. to antifungals is mainly focused on azole antifungals. The research progress on antifungal resistance of pathogenic Aspergillus spp., including the diagnosis for resistance and the molecular mechanisms, such as over－expression of efflux pumps, mutations in the target enzyme (Cyp51), formation of biofilm and heat shock protein 90 (Hsp90)－mediated signaling pathways are reviewed.