Eukaryotic expression vector containing different proteins (C, M, E and NS1-­5) of dengue virus serotype 2 (DENV­-2) were constructed and intramuscularly injected directly to mice for developing antibodies against DENV-­2. Two weeks after the fourth immunization, sera were collected and the immune effects were evaluated by enzyme­linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA) and Western blotting. Specific antibodies could be obtained by DNA immunization, and the titers of these antibodies fluctuated between 1:400­ - 1:16 127. Of those, the antibody against protein E showed the highest titer at 1:16 127 whereas the antibodies against proteins NS3, NS4b and NS5 had the lowest titer at 1:400. The results showed that all antibodies acquired by DNA immunization could produce specific immunofluorescent reactions when tested using DENV­-2-infected Vero cells, suggesting that antibodies prepared by DNA immunization could specifically identify naturally infected DENV­-2. Antibodies against proteins E, NS1, NS4b and NS5 could also identify heat­denatured proteins. The results generated from this study provided the base for further research.