Helicobacter pylori (H. pylori) is a chronic infectious pathogen with high prevalence. H. pylori infection is closely related to chronic gastritis, dyspepsia, iron deficiency, anemia and growth retardation in children. It is a lifetime health issue for children. Therefore, finding a safe, efficient and convenient detection method suitable for children is very important. Currently, the commonly used detection methods in children include rapid urease test (RUT), histological examination, bacterial culture, urea breath test (UBT), serum H. pylori­Ig-IgG antibodies, H. pylori stool antigen, etc. In general, a non­invasive, efficient, operation friendly and economic H. pylori detection method is needed. Therefore suitable method should be chosen according to the diagnosis purpose. This review summarizes various detection methods and their clinical value for H. pylori infection in children.