To investigate the situation of environmental pollution by avian influenza virus in Hubei Province, 2013-2014, 1 642 live-bird related environmental samples collected from 7 monitoring points were detected by real-time polymerase chain reaction (PCR). The virus types and positive rates were analyzed. The results showed that the positive rates of avian influenza virus in 2013 and 2014 were 16.8% and 21.4%, respectively. The leading virus subtype was H9 (57.3%). H7 avian influenza virus was not detected. The positive rates in different areas and seasons were different. The positive rate of northwest in Hubei Province was higher than that of southeast and Jianghan Plain. The poultry dressing plant had the highest detection rate. The sample with highest positive rate was from poultry cleaning wastewater. The results suggest that the environment of Hubei Province has high detection rate of avian influenza virus, with various types. The people who work in poultry dressing plants have higher risk. Therefore health education should be strengthened to enhance the personal protection consciousness.