In photodynamic therapy (PDT), the reactive oxygen species generated by the activation of photosensitizers by light of an appropriate wavelength, are able to inactivate microbes by damaging various microbial components. Due to its multi-targeting antimicrobial capability, PDT has shown a potential as an effective alternative therapeutic option against methicillin-resistant Staphylococcus aureus (MRSA). In this study, MRSA strains isolated from burn, acute pharyngitis, nasosinusitis and pneumonia patients were subjected to a 5-aminolevulinic acid (ALA) mediated light emitting diode (LED) array in vitro. The results showed that a significant reduction (1.8 log₁₀ cfu) was achieved with an ALA treatment (1 h, 5 mmol/L) followed by 1 h of illumination [(633±10) nm wavelength light source, and a 360 J/cm² light dose]. MRSA strains collected from different tissues showed a similar sensitivity to the treatment.