To analyze the risk factors of infections caused by linezolid-resistant Enterococcus faecalis (E. faecalis), 165 cases from Shenzhen Nanshan District People’s Hospital with E. faecalis infection recorded from January 2010 to September 2015 were studied. The patients were divided into linezolid-sensitive group and linezolid-resistant/intermediate group. E. faecalis mainly came from urine culture (53.94%), and then wound secretion culture (21.82%) and bloodstream culture (6.06%). E. faecalis infection was mainly found in Department of Urology (35.76%) and Department of Hepatobiliary Surgery (9.70%). Both univariate analysis and logistic regression analysis showed that carbapenem exposure and indwelling catheter were associated with linezolid-resistant E. faecalis infections. The results suggest that the clinical application of carbapenems should be strictly controlled, and the management of nosocomial infection should be strengthened.