Experimental course is an essential and indispensable part of modern medical microbiology teaching. Unfortunately, in the reform of experiment teaching of medical microbiology, we often overlook the importance of the preparation of experimental course in fulfilling high-quality teaching. In this paper, we reviewed the status of medical microbiological experimental course in three stages: before 2000, 2000-2010 and 2011-present. Then we analyzed and discussed on the considerations in the preparation of experimental courses as well as the requirements of experimental conditions, and suggested that highly competent technicians play an important role in experiment teaching of medical microbiology.