Abstract: Objective: To investigate the correlation between exoU gene expression in Pseudomonas aeruginosa and lasI gene deletion and drug resistance. Methods: 640 strains of Pseudomonas aeruginosa (PA) were collected and isolated in our hospital from January 2020 to December 2020, and PCR was used to detect the distribution of exoU virulence genes from different sources of strains, and fluorescence quantitative PCR technology was used to observe the expression of transcriptional regulation related gene exoU and the expression of lasI gene and downstream virulence genes in the strain; The agar dilution method was used to detect the sensitivity of antimicrobial drugs; And Pearson correlation was used to analyze the relationship between the relative expression of each gene. Results: The distribution of 640 PA specimens was mainly sputum (51.56%), blood (22.19%) and pus (14.69%). The exoS+/exoU- gene combination has the highest detection rate in PA samples from all sources, and its detection rates in sputum, blood and pus are 56.97% (188/330), 71.83% (102/142) and 67.01, respectively %(65/97); drug sensitivity results show that 640 strains of PA are resistant to 8 kinds of antibacterial drugs to varying degrees, and the sputum-derived PA strain is more resistant to imipenem than blood, pus and other sources Strain (P<0.001). PA carrying exoU gene has a higher resistance rate to imipenem, aminoglycosides, fluoroquinolones, and macrolide antibacterials (P<0.001); PA carrying exoS gene has a higher resistance to aztreonam Resistance (P<0.05); The resistance of PA with lasI gene deletion to various antibacterial drugs was significantly reduced (P<0.001), and there was a significant negative correlation between the relative expression of the transcriptional regulation-related gene ptrA of the PA strain carrying the exoU gene and exsA and the effector gene exoU (r=-0.645, P<0.001; r=-0.587, P<0.001); There was also a significant negative correlation between the relative expression of the transcriptional regulation-related gene ptrA of the PA strain carrying exoS gene and exsA and the effector gene exoS (r=-0.618, P<0.001; r=-0.532, P<0.001). After lasI gene deletion, the expression levels of downstream virulence genes lasA and aprX decreased significantly (P <0.001), and there was a significant positive correlation between the expression levels of lasA and aprX and the relative expression of lasI gene (r=0.489, P < 0.001; r=0.512, P <0.001). Conclusion: There is a significant correlation between the expression of exoU gene and the drug resistance of Pseudomonas aeruginosa. The expression of virulence genes such as exoU and drug resistance may be related to the negative regulatory gene ptrA; The deletion of the lasI gene will significantly inhibit the expression of virulence genes in the QS system of Pseudomonas aeruginosa and the drug resistance of the bacteria.
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References
参考文献
[1]Matsui H , Sano Y , Ishihara H , et al. Regulation of pyocin genes in Pseudomonas aeruginosa by positive (prtN) and negative (prtR) regulatory genes.[J]. J Bacteriol, 2019, 175(5): 1257-1263.
[2]Leinweber A , Weigert M , Kümmerli, Rolf. The bacterium Pseudomonas aeruginosa senses and gradually responds to interspecific competition for iron[J]. Evolution, 2018, 72(7): 1515-1528.
[3]Emily, A, Williams, et al. Fitting pieces into the puzzle of Pseudomonas aeruginosa type III secretion system gene expression.[J]. J Bacteriol, 2019, 201(13):e00209-19.
[4]Zhang Y , Hu L , Qiu Y , et al. QsvR integrates into quorum sensing circuit to control Vibrio parahaemolyticus virulence[J]. Environ Microbiol, 2019, 21(3): 1054-1067.
[5]尚红, 王毓三, 申子瑜. 全国临床检验操作规程[M]. 人民卫生出版社, 2015, 124-128.
[6]Gambari R, Brognara E, Spandidos DA, et al. Targeting oncomiRNAs and mimicking tumor suppressor miRNAs: new trends in the development of miRNA therapeutic strategies in oncology (Review)[J]. Int J Oncol, 2016, 49(1): 5-32.
[7]Véronique, Andrey, Pierre-Alex, et al. Impact of co-amoxicillin-resistant Escherichia coli and Pseudomonas aeruginosa on the rate of infectious complications in paediatric complicated appendicitis.[J]. Swiss medical weekly, 2019, 149(15):w20055.
[8]方雪瑶, 胡龙华, 杭亚平,等. 铜绿假单胞菌Ⅲ型分泌系统相关毒力基因在抗菌药物中表达差异的研究[J]. 中国抗生素杂志, 2019, 44(12):1390-1393.
[9]赵芝静, 刘心伟, 张小倩,等. 铜绿假单胞菌生物被膜调控机制的研究进展[J]. 中华预防医学杂志, 2020, 54(12):1469-1472.
[10]张秀彩, 张肖肖, 王忠永,等. 铜绿假单胞菌中T3SS毒力基因分布、表达及与抗菌药物耐药相关性研究[J]. 临床检验杂志, 2019, 037(1):14-18.
[11]Antitoxin HigA inhibits virulence gene mvfR expression in Pseudomonas aeruginosa[J]. Environ Microbiol, 2019, 21(8): 2707-2723.
[12]Mccarthy K L , Wailan A M , Jennison A V , et al. P. aeruginosa blood stream infection isolates: A "full house" of virulence genes in isolates associated with rapid patient death and patient survival[J]. Microb Pathog, 2018, 119(15): 81-85.
[13]陈蓉, 张方圆, 严杰. 不同疾病标本中铜绿假单胞菌分离鉴定与多重耐药性分析[J]. 中国人兽共患病学报, 2019, 35(3):239-244.
[14]穆丽丽, 牛犇, 赵勇. 副溶血性弧菌分泌系统在致病力中作用的研究进展[J]. 微生物学报, 2019, 59(4):37-47.
[15] Song Y Q, Yang C, Chen G K, et al. Molecular insights into the master regulator CysB-mediated bacterial virulence in Pseudomonas aeruginosa.[J]. Mol Microbiol, 2019, 111(5):1195-1210.
[16]Kelei, Zhao, Linjie, et al. Behavioral heterogeneity in quorum sensing can stabilize social cooperation in microbial populations.[J]. Bmc Biol, 2019, 17(1): 20-25.
[17]税剑, 邹明祥, 王海晨,等. 铜绿假单胞菌临床分离株生物膜,群体感应相关基因及耐药性分析[J]. 临床检验杂志, 2017, 35(4):254-257.
[18]鄂顺梅, 陈富, 龙一飞,等. LasI/rhlI基因缺失和阿奇霉素对铜绿假单胞菌毒力基因的影响[J]. 实用医学杂志, 2018, 34(16):2773-2776.
[19]曾婷, 曾凌, 曹先伟,等. 耐碳青霉烯类铜绿假单胞菌耐药基因与药敏分析[J]. 中华医院感染学杂志, 2019, 29(20):33-37.
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