Neutrophils can capture and kill pathogens by releasing neutrophil extracellular trap (NET) during anti-infection immunity. NET is a double-edged sword. On the one hand, it can maintain the homeostasis of the body; on the other hand, excessive NET or delayed removal may lead to the occurrence of some diseases. The mechanisms by which bacteria induce and resist NET are different. This article reviews the formation and release of NET and their roles in bacterial infection.