The present paper reported a case of multiple severe skin infection caused by P. wickerhamii. By culturing the deep skin tissue at the erosion site of the patient's right hand back, white dry colonies could be seen growing on the blood plate after 48 hours of cultivation at 35 ℃ and 5% CO2. Purple spherical or oval shaped bacteria bodies could be seen under the gram staining oil microscope. Light purple creamy colonies were observed on the candida colorimetric plate. After staining with lactophenol cotton blue and calcofluor white, endogenous spores resembling mulberry embryos could be seen under the oil microscope. Finally, the results of the VITEK 2-Compact fully automatic microbial identification analysis system and VITEK MS mass spectrometer identification were P. wickerhamii. The patient was given voriconazole and itraconazole for antifungal treatment based on his condition and drug sensitivity results. Luckily enough, the patient's rash improved significantly and was discharged smoothly.