The study on molecular characteristics and whole genome typing of salmonella

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Journal of Microbes and Infections ›› 2024, Vol. 19 ›› Issue (2) : 65-71.

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Abstract: The purpose of this study is to understand the molecular characteristics of Salmonella, reveal the genetic relationship among the strains, and provide a certain basis for the origin of Salmonella outbreak. Virulence and drug resistance genes of 105 Salmonella strains isolated from Changchun in recent years had been screened, by whole genome sequencing and bioinformatics methods. The wgSNP, MLST and cgMLST genotyping were performed for 105 Salmonella. 144 virulence genes were analyzed on this study. The results showed that the gene of Salmonella Pathogenicity Island 1 (SPI) was relatively stable.The carrying rate of the virulence factors outside the secretion varied greatly with serotype.69 drug resistance genes of Classes were screened, with high carrying rate of Aminoglycosides (100%) , β-lactams(73.33%) , Sulfonamides (72.38%), and low carrying rate of Macrolides (6.67%) and Lincosamides (0.95%) . The cgST and MLST types of 105 strains of salmonella were identical and divided into 13 species. The major prevalent strains, Salmonella enteritidis (ST11) and Salmonella I 4, [5] , 12: I:-(ST34) , were essentially in the same branch as the international prevalent strains. This study confirmed that Salmonella carries stable virulence genes and multi-drug resistance genes in Changchun. The major prevalent strains were distributed in different time and source. The molecular characteristics of the two strains were consistent with those of the foreign prevalent strains. The serotypes were closely related to ST and cgST types.

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The study on molecular characteristics and whole genome typing of salmonella[J]. Journal of Microbes and Infections. 2024, 19(2): 65-71

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