Objective To analyze the infection subtype distribution of Human papillomavirus (HPV) among women in Suzhou area, and to describe the cervical cytology findings in HPV-positive women, providing a theoretical basis for the prevention and treatment of HPV infection in Suzhou. Methods Fluorescence quantitative polymerase chain reaction (qPCR) was used to detect 2852 cervical exfoliated cell specimens from women at Affiliated Suzhou Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, and its medical community unit, Tong'an Community Health Center, Suzhou High-tech Zone. from 2020 to 2021. The total HPV infection, age-specific infection characteristics, and genotype distribution were analyzed. Liquid-based cytology (TCT) was performed for all HPV-positive cases and classified according to TBS 2014. Results Among 2852 female subjects, 502 were HPV positive, with an infection rate of 17.60% (502/2852). Of these, 447 cases (89.04%) were single infections, and 55 cases (10.96%) were multiple infections. The age-specific infection rates were: 164 cases (5.75%) in 34-39 years old, 91 cases (3.19%) in 40-49 years old, 182 cases (6.38%) in 50-59 years old, and 65 cases (2.28%) in 60-65 years old. The top three HPV subtypes detected were: HPV52 (22.51%, 113/502), HPV58 (13.35%, 67/502), and HPV16 (9.76%, 49/502). Among 502 HPV-positive women, there were 71 cases of Atypical squamous cells of undetermined significance (ASC-US), 19 cases of Low-grade squamous intraepithelial lesion (LSIL), 1 case of High-grade squamous intraepithelial lesion (HSIL), and 1 case of Atypical squamous cells cannot exclude HSIL (ASC-H). Conclusion The HPV positive rate among women in Suzhou area varies with age groups. The main infection types are high-risk subtypes and single infections, with HPV52, HPV58, and HPV16 being the predominant subtypes.