The infection and drug sensitivity of Mycoplasma hominis (Mh) and Ureaplasma urealyticum (Uu) in outpatient and inpatient female patients in the Wusong area of Baoshan, Shanghai were studied to provide a scientific evidence for clinical diagnosis and treatment. Mycoplasma culture identification and count drug sensitivity kits were used to carry out Mycoplasma culture, identification and drug susceptible test. A total of 413 cases underwent Mycoplasma culture and drug sensitivity test, of which 178 cases were positive for mycoplasma culture (43.10%), 17 cases with simple Mh infection (4.12%), 152 cases with simple Uu infection (36.80%), and 9 cases with mixed infection (2.18%). In simple Mh infection, the top five high-sensitivity rates from high to low are: doxytetramycin/minocycline, josamycin, clindamycin and gatifloxacin. There is no significant difference in the sensitivity rates of the first three drugs (P>0.05). There is a significant difference between doxytetramycin/minocycline and clindamycin or gatifloxacin (P<0.05, P<0.01). The top five drug resistance rates from low to high are: doxytetramycin/minocycline, josamycin, gatifloxacin, thiamphenicol, and there is no significant difference in the resistance rate of the five drugs (P>0.05). In simple Uu infection, the top five high-sensitivity rates from high to low are: doxytetramycin/minocycline, josamycin, azithromycin and clarithromycin. The difference between the first three drugs has no statistical significance (P>0.05). The difference between doxytetramycin/minocycline and azithromycin or clarithromycin is statistically significant (P<0.01). The drug resistance rates from low to high are: doxytetramycin/minocycline, josamycin, gatifloxacin, azithromycin/erythromycin/levofloxacin, and the resistance rate of doxytetramycin/minocycline is not statistically different from the latter two drugs (P>0.05), and is statistically different from azithromycin/erythromycin/levofloxacin (P<0.05). When treating mycoplasma infections of the reproductive tract, the clinicians should try to choose minocycline, doxytetramycin or josamycin. The alternative drugs for simple Mh and Uu infections are clindamycin and azithromycin respectively.