Salmonella Poona is a rare serotype of Salmonella. This study was conducted on a Salmonella strain isolated from the fecal sample of a patient reported by the active surveillance project of foodborne diseases in Changchun Children’ Hospital. Bacteriological identification, serotyping, drug sensitivity testing, and whole genome sequencing were employed to investigate its biological and molecular profiles. Results demonstrated that this strain was biochemically confirmed as Salmonella and identified as Salmonella Poona by the serological typing. The isolate exhibited resistance to most antibiotics and harbored a diverse array of resistance and virulence genes. This research confirms the first isolation of a multidrug-resistant and highly virulent Salmonella Poona strain in Changchun City, highlighting the need for heightened attention to this pathogen. This study aimed to analyze the biological characteristics and molecular features of Salmonella Poona to provide scientific evidence for clinical treatment and prevention.