The present study aims to establish a method for rapid detection of a panel of pathogenic viruses based on reverse transcription-loop-mediated isothermal amplification (RT-LAMP). Primer sets of 10 pathogenic viruses with high specificity were screened. It was suggested that the method was safe, rapid, convenient, and high-throughput in detection of pathogenic viruses. When combined with the new immobilized reaction tubes, the amplification for 10 pathogenic viruses in 1-100 copies could be finished within 45 min. In the assay of 30 serum samples, one rift valley fever virus-positive sample was obtained. A rapid, convenient, low-cost, real-time and high-throughput detection method for common pathogenic viruses was established. The results indicate that RT-LAMP method for 10 high-pathogenic viruses has a good application prospect in rapidly screening suspected patients in areas with high population mobility such as ports.