Ureaplasma urealyticum (U. urealyticum) is an opportunistic pathogen. At present, the study of its resistance mechanisms mainly includes the detection of the related drug-resistance mutation sites for quinolones, macrolides and tetracyclines, and the influence of biofilm on the drug susceptibility. In recent years, some researchers have applied multilocus sequence typing (MLST) technology to explore the drug-resistance sequence types of U. urealyticum. Rapid identification of drug-resistant strains is an urgent clinical need for rational choice of antimicrobial agents.