Journal of Microbes and Infections >
Tandem expression of four avian influenza M2e polypeptides and effects of oral immunization in mice
Received date: 2018-05-17
Online published: 2018-01-01
In order to develop a universal oral vaccine for bird flu, four avian influenza virus-specific matrix protein 2 ectodomain (M2e) polypeptides were serially ligated with the mucosal immune adjuvant CTA1-DD, expressed in prokaryotic cells and purified as CTA1DD-AVI4M2e fusion protein. The BALB/c mice were orally immunized with the fusion protein CTA1DD-AVI4M2e. The results showed that the intestinal fluid IgA antibody titer and serum IgG antibody titer in the experimental group were significantly higher than those in the control group. The specific IgG antibody titer in the serum was about 360 times；in the first, second, and third segmented intestinal fluid, the IgA antibody titers were about 360 times, 120 times and 9 720 times, respectively. The results demonstrate that CTA1DD-AVI4M2e has a significant oral immune effect, providing the basis for further studies.
LUO Bin1 , 3 , QIAN Kai1 , 3 , XU Wanxiang2 , TANG Haiping2 , JI Chaoneng1 , 3 , XIE Yi1 , 3 , GU Shaohua1 , 3 . Tandem expression of four avian influenza M2e polypeptides and effects of oral immunization in mice[J]. Journal of Microbes and Infections, 2018 , 13(4) : 220 -226 . DOI: 10.3969/j.issn.1673-6184.2018.04.005
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