Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus

YANG Han1; HUANG Kaifeng1; XU Su2; ZHAO Huanqiang1; XU Xiaogang2; HU Fuping2; HE Chunyan1; Gong Fang3; LIU Qingzhong1

doi:10.3969/j.issn.1673-6184.2019.01.004

Journal of Microbes and Infections >

2019 , Vol. 14 >Issue 1: 23 - 29

DOI: https://doi.org/10.3969/j.issn.1673-6184.2019.01.004

Original Article

Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus

YANG Han1 ,
HUANG Kaifeng1 ,
XU Su2 ,
ZHAO Huanqiang1 ,
XU Xiaogang2 ,
HU Fuping2 ,
HE Chunyan1 ,
Gong Fang3 ,
LIU Qingzhong1

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1. Department of Clinical Laboratory, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China; 2. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China; 3. Department of Clinical Laboratory, The Third Hospital Affiliated to Nantong University, Nantong 226000, Jiangsu Province, China

Received date: 2018-09-25

Online published: 2019-02-25

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Abstract

This study aimed to explore the optimal culture conditions for transcriptional expression of leukocidin ED (lukED) of Staphylococcus aureus (S. aureus). The transcription level of lukE in S. aureus strain Newman was detected at different growth phases (early-exponential, middle-exponential, late-exponential and stationary phases) in TSB, BHI, YCP, MHB and LB media by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Based on the medium mediating the lowest transcription level of lukE, the different compositions of medium producing the highest mRNA level of lukE was added to discover the substances promoting lukED expression. The findings showed that lukE mRNA level was highest at the late-exponential phase in YCP medium, in which yeast extract, casamino acids and sodium pyruvate could all strikingly increase the expression of lukE, with the first two being better.

Key words： Staphylococcus aureus; Leukocidin ED; mRNA level; Growth phase; Medium

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YANG Han1 , HUANG Kaifeng1 , XU Su2 , ZHAO Huanqiang1 , XU Xiaogang2 , HU Fuping2 , HE Chunyan1 , Gong Fang3 , LIU Qingzhong1 . Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus[J]. Journal of Microbes and Infections, 2019 , 14(1) : 23 -29 . DOI: 10.3969/j.issn.1673-6184.2019.01.004

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