The change of endogenous siRNAs in coxsackie virus type B3-infected HeLa cells

YAO Hailan1; SONG Juan2; WANG Xinling2; WANG Ruifang2; SONG Qinqin2; SHI Bintian2; HAN Jun2

doi:10.3969/j.issn.1673-6184.2020.02.005

Journal of Microbes and Infections >

2020 , Vol. 15 >Issue 2: 98 - 103

DOI: https://doi.org/10.3969/j.issn.1673-6184.2020.02.005

Original Article

The change of endogenous siRNAs in coxsackie virus type B3-infected HeLa cells

YAO Hailan1 ,
SONG Juan2 ,
WANG Xinling2 ,
WANG Ruifang2 ,
SONG Qinqin2 ,
SHI Bintian2 ,
HAN Jun2

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1. Department of Biochemistry & Immunology, Capital Institute of Pediatric, Beijing 100020, China; 2. State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received date: 2019-09-02

Online published: 2019-04-25

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Abstract

To explore whether coxsackie virus type B3 (CVB3) infection can induce the expression of endogenous siRNA, siRNAs were analyzed in CVB3-infected HeLa cells at 3h and 6h post-infection (pi) by the high-throughput sequencing. The results showed that CVB3 induced 14 endogenous siRNA and 5 endogenous siRNA in HeLa cells at 3 h and 6 h pi, respectively. Two sustainably expressed endogenous siRNAs were further confirmed at 3 h and 6 h pi using quantitative PCR. Results of sequence homology alignment showed that two siRNAs could recognize the precursor RNA of 45S ribosome and 28S ribosome. The results suggest that CVB3 infection may interfere ribosome maturation.

Key words： Coxsackie virus type B3; HeLa cell; Endogenous siRNA

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YAO Hailan1 , SONG Juan2 , WANG Xinling2 , WANG Ruifang2 , SONG Qinqin2 , SHI Bintian2 , HAN Jun2 . The change of endogenous siRNAs in coxsackie virus type B3-infected HeLa cells[J]. Journal of Microbes and Infections, 2020 , 15(2) : 98 -103 . DOI: 10.3969/j.issn.1673-6184.2020.02.005

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