To understand the quantity, species, and drug sensitivity of bacteria in the external auditory canal of healthy people with wet ear, the secretions of both right and left ears were collected from 38 healthy wet ear people without ear diseases. A total of 76 samples were subjected to standard clinical culture and isolation procedure on LB plates. The total number of colonies were counted, and representative colonies were subjected to species identification (VETEK-2) and drug sensitivity assays (Kirby-Bauer test). The beta-lactamase of penicillin resistant strains was detected by method of cefnithiphene disk. The results showed that the bacterial number in 88.3% of samples were within 1×10² CFU/sample～1×10⁶ CFU/sample. 20 species of bacteria were detected, 97.1% were cocci and 2.9% were bacilli. The top four bacteria were Staphylococcus cephalus, Staphylococcus auriculae, Staphylococcus hominus and Staphylococcus epidermidis. The sensitivity rate to tobramycin, minocycline, vancomycin and furantoin were more than 90.0%, and resistant rate to cotrimoxazole and penicillin was 50%; 25 of 132 Staphylococcus strains were resistant to penicillin and the positive rate of beta-lactamase was 44.0%.