Soluble prokaryotic expression of varicella-zoster virus glycoprotein E without N-terminal signal peptide and its immunogenicity evaluation

WEI Mingtong1; XIA Bingbing2; HE Zhiyuan2; ZHOU Wei2; LIU Xingdong3; ZHAO Jun2; 4; CHEN Jingxian4; WANG Mingli2; 4

doi:10.3969/j.issn.1673-6184.2020.06.006

2020 , Vol. 15 >Issue 6: 377 - 384

DOI: https://doi.org/10.3969/j.issn.1673-6184.2020.06.006

Original Article

Soluble prokaryotic expression of varicella-zoster virus glycoprotein E without N-terminal signal peptide and its immunogenicity evaluation

WEI Mingtong1 ,
XIA Bingbing2 ,
HE Zhiyuan2 ,
ZHOU Wei2 ,
LIU Xingdong3 ,
ZHAO Jun2 ,
4 ,
CHEN Jingxian4 ,
WANG Mingli2 ,
4

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1. The First Clinical Medical College of Anhui Medical University, Hefei 230032, Anhui Province, China; 2. Wuhu Interfell Research Institute, Wuhu 241000, Anhui Province, China; 3. Second Clinical Medical College of Anhui Medical University, Hefei 230032, Anhui Province, China; 4. Institute of Clinical Virology, Anhui Medical University, Hefei 230000, Anhui Province, China

Received date: 2020-01-02

Online published: 2020-12-25

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Abstract

Varicella zoster virus (VZV) glycoprotein E (gE) is the main candidate protein for VZV subunit vaccine. Unfortunately, the expression of gE in prokaryotic expression was mainly found in the inclusion body. In this study, a deletion of N-terminal 30 amino acids of gE was constructed on pET32a, expressed in BL21 (DE3). The soluble gE was purified and used to make polyclonal antibodies in BALB/c mice. The titer and specificity of polyclonal antibodies were determined by enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence. The results showed that the soluble recombinant gE produced by BL21/pET32a-VZV gE system is a practical way for vaccine development.

Key words： Varicella zoster virus; Glycoprotein E; Prokaryotic expression; Polyclonal antibody; Evaluation of immune

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WEI Mingtong1 , XIA Bingbing2 , HE Zhiyuan2 , ZHOU Wei2 , LIU Xingdong3 , ZHAO Jun2 , 4 , CHEN Jingxian4 , WANG Mingli2 , 4 . Soluble prokaryotic expression of varicella-zoster virus glycoprotein E without N-terminal signal peptide and its immunogenicity evaluation[J]. Journal of Microbes and Infections, 2020 , 15(6) : 377 -384 . DOI: 10.3969/j.issn.1673-6184.2020.06.006

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