ISSN 1673-6184 CN 31-1966/R
Objective To test a novel baculovirus expressing Epstein-Barr virus (EBV) lytic replication immediate early gene Rta, in combination with antiviral chemical ganciclovir, for the activation of latent EBV in nasopharyngeal carcinoma (NPC) cells, and the killing of tumor cells. Methods A baculovirus vector with EBV replication origin OriP, and the Rta expression cassette led by CMV promoter, was used to treat EBV-latently infected NPC cell line Hone1-EBV, as well as nude mice tumor model established from the cell line, in combination with ganciclovir. The effect on the growth of cell and tumor was studied. Results When treated with recombinant baculovirus in combinantion with ganciclovir, the growth of EBV-positive cells was significantly reduced to only 51% of the control. In the nude mice experiments, tumor growth was also greatly reduced. At 10th day after the first injection, the average volume and weight of the treated group was only about 20% and 30% of that of the control group, respectively. Histological analysis indicated there was significant cell necrosis in the baculovirus-ganciclovir-treated tumor tissue. Conclusion Co-application of RTA-expressing baculovirus vector and gancyclovir may be useful for the therapy of EBV associated NPC.
Studies on hepatitis B virus genotypes and responses to antigen-antibody complex therapeutic vaccine
Objective To study whether there are differences in the responses of chronic hepatitis B patients infected with different hepatitis B virus (HBV) genotypes to HBsAg-HBIG therapeutic vaccine. Method Serum samples from sixty seven chronic hepatitis B patients who completed the full course of immunization with 60?g HBsAg-HBIG (YIC) were assayed for their virus genotypes by PCR-RFLP, in association with their viral and HBeAg sero-conversion responses to YIC treatment. Results Among the 67 patients, 22 were infected with HBV genotype B, and 43 were infected with genotype C, while in 2 patients the genotype of HBV could not be identified. In association with the responses to YIC treatment, no significant therapeutic differences were found between patients who were infected with HBV genotype B or with HBV genotype C. Conclusion Infections with HBV genotypes B or C do not affect the therapeutic responses in chronic hepatitis B patients to YIC.
Objective To establish the HHV-6-specific CD4+ T cell clones and further study the character of HHV-6-specific CD4+ T cells. Methods We acquired CD4+ T cell clones by the liquid microwell limiting dilution technique, and selected HHV-6-specific CD4+ T cell clones by means of 3H-thymidine uptake. The phenotype of HHV-6-specific T cell clones were analyzed with FACS. We tested the IL-10, IL-4 or IFN-γ secretion from HHV-6-specific CD4+ T cell clones by ELISA. Results Of the five HHV-6-specific CD4+ T cell clones, four showed proliferative responses to the stimulation with HHV-6 infected JJHAN lysates. Moreover, proliferation responses of HHV-6-specific CD4+T cell clones depended on the concentration of the HHV-6-infected JJHAN lysates. The phenotypic analysis with FACS demonstrated that HHV-6-specific T cell clones consisted of CD3+ CD4+ T cells. CD4+ T cell clones-W-2 and W-4 possessed the feature of high levels of IL-10 production, while CD4+ T cell clones-W-1 possessed the feature of high levels of IL-10 and IFN-γ production and W-3 possessed the feature of high levels of IFN-γ production. Conclusions The HHV-6-specific CD4+ T cell clones, were established in our study so as to further study their pneotype, the character of cytokine secrection, and to provide experimental basis for selecting HHV-6-specific CD4+ Treg cell clones.
Objective To seek better diagnostic tests for tuberculous pleural effusion. Methods Adenosine deaminase (ADA) and anti-TB antibody in pleural effusion were tested by modified Martineck’s method and ELISA in 50 cases of tuberculous pleural effusion, 45 cases of malignant pleural effusion and 30 cases of right cardiac insufficiency pleural effusion, respectively. Result The level of ADA and anti-TB antibody in pleural effusion remarkably higher in tuberculous pleural effusion than in malignant pleural effusion and right cardiac insufficiency pleural effusion (p<0.01). When ADA >45U/L and positive anti-TB antibody were present，their sensitivity, specificity and accuracy in diagnosis of tuberculous pleural effusion are 100%, 98.6%, 99.2% and 90% , 93.3%, 92%, respectively. Conclusion The tests of ADA and anti-TB antibody in pleural effusion are very important in diagnosis tuberculous pleural effusion.
