Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus
YANG Han1, HUANG Kaifeng1, XU Su2, ZHAO Huanqiang1, XU Xiaogang2, HU Fuping2, HE Chunyan1, Gong Fang3, LIU Qingzhong1
1. Department of Clinical Laboratory, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China; 2. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China; 3. Department of Clinical Laboratory, The Third Hospital Affiliated to Nantong University, Nantong 226000, Jiangsu Province, China
Abstract：This study aimed to explore the optimal culture conditions for transcriptional expression of leukocidin ED (lukED) of Staphylococcus aureus (S. aureus). The transcription level of lukE in S. aureus strain Newman was detected at different growth phases (early-exponential, middle-exponential, late-exponential and stationary phases) in TSB, BHI, YCP, MHB and LB media by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Based on the medium mediating the lowest transcription level of lukE, the different compositions of medium producing the highest mRNA level of lukE was added to discover the substances promoting lukED expression. The findings showed that lukE mRNA level was highest at the late-exponential phase in YCP medium, in which yeast extract, casamino acids and sodium pyruvate could all strikingly increase the expression of lukE, with the first two being better.
杨涵1，黄凯峰1，徐溯2，赵焕强1，徐晓刚2，胡付品2，何春燕1，龚芳3，刘庆中1. 影响金黄色葡萄球菌杀白细胞素ED转录表达的培养条件[J]. 微生物与感染, 2019, 14(1): 23-29.
YANG Han1, HUANG Kaifeng1, XU Su2, ZHAO Huanqiang1, XU Xiaogang2, HU Fuping2, HE Chunyan1, Gong Fang3, LIU Qingzhong1. Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus. JOURNAL OF MICROBES AND INFECTIONS, 2019, 14(1): 23-29.
2019, Vol. 14 
