Abstract:The present paper aimed to study the expression of CT703 mRNA of Chlamydia trachomatis( Ct) serovars L2 under acute infection or persistent infection conditions in HeLa cells. Transmission electron microscopy ( TEM) was used to determine the establishment of acute infections and persistent infections. The expression of CT703 mRNA of Ct in HeLa cells was detected by reverse transcriptase-polymerase chain reaction ( RT-PCR) . Giemsa staining showed that there were enlarged and aberrant reticulate bodies ( RBs) in inclusions in persistent infections. The expression of CT703 mRNA of Ct in persistent infections was significantly lower than that in acute infections, which may be one of the mechanisms underlying the persistent infection of Ct in cells.
马淑慧; 王芙艳; 霍治; 杜昆; 罗奇志; 余平. 沙眼衣原体基因CT703在HeLa细胞中的表达[J]. 微生物与感染
, 2009, 4(4): 217-222 .
MA Shu-Hui; WANG Fu-Yan; HUO Zhi; DU Kun; LUO Qi-Zhi; YU Ping. Study on the expression of CT703 mRNA of Chlamydia trachomatis in
HeLa cells. Journal of Microbes and Infections, 2009, 4(4): 217-222 .