Clinical analysis of Chlamydia trachinomatis urogenital tract infection in patients with sexually transmitted diseases via real-time fluorescent polymerase chain reaction
YOU Yong-Yan, GONG Kuang-Long, GONG Xiang-Dong,SHA Zhong, ZHANG Jin-Ping, SUN Hou-Hua
Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
Abstract：The clinical and laboratory analysis of 380 urogenital tract secretion samples from sexually transmitted diseases patients for Chlamydia trachomatis detection was performed by polymorphonuclear leukocyte count and fluorescent real-time polymerase chain reaction (PCR). The results showed that of 216 male patients 92.6% presented urethral polymorphonuclear leukocyte count ≥5, and 62 cases (28.70%) were Chlamydia trachomatis-positive detected by PCR. There were 30 case with Chlamydia trachomatis and Neisseria gonorrheae coinfection (13.98%). Of 164 female patients, 53.0% presented cervical canal polymorphonuclear leukocyte count ≥10, and 33 cases (20.1%) were Chlamydia trachomatis-positive detected by PCR. The results indicate that the application of real-time fluorescent PCR can improve positive detection rate of Chlamydia trachomatis and control its spread in sexually transmitted disease clinics.
尤永燕，龚匡隆，龚向东，沙仲，张津萍，孙厚华. 实时荧光聚合酶链反应检测性病患者泌尿生殖道沙眼衣原体的临床分析[J]. 微生物与感染
, 2013, 8(3): 153-156.
YOU Yong-Yan, GONG Kuang-Long, GONG Xiang-Dong,SHA Zhong, ZHANG Jin-Ping, SUN Hou-Hua . Clinical analysis of Chlamydia trachinomatis urogenital tract infection in patients with sexually transmitted diseases via real-time fluorescent polymerase chain reaction. Journal of Microbes and Infections, 2013, 8(3): 153-156.
