Coxsackievirus B3 2A protease blocks nuclear translocation of nuclear factor κB (NF-κB) and inhibits dimerization of NF-κB
SHENG Lin-Jun, SONG Qin-Qin, LU Ming-Zhi, SONG Juan, SUN Peng, WANG Bao-Dong, CHI Miao-Miao, HAN Jun
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China Center for Diseases Control and Prevention, Beijing 102206, China
Abstract:To study the impact of coxsackievirus B3 2A protease on nuclear factor κB (NF-κB ), BHK cells were transfected with a plasmid pcDNA3.1-2A expressing CVB3 2A protease along with pGL3-NF-κB -promotor-luc. The activity of luciferase was detected to identify the relative effect of NF-κB upon tumor necrosis factor α (TNF-α) stimulation. The dimerization of NF-κB /p65 were also detected with Western blotting and translation of NF-κB /p65 was observed by immunofluorescence. The results showed that CVB3 2A protease not only inhibited the expression of luciferase activities from NF-κB /p65 promoter but also interfered the nuclear translocation of NF-κB /p65. CVB3 2A protease still inhibited the dimerization of NF-κB /p65. It is concluded that CVB3 protease 2A inhibits the activation of NF-κB pathway.
盛琳君,宋芹芹,卢明枝,宋娟,孙鹏,王宝栋,迟苗苗,韩俊. 柯萨奇病毒B3蛋白酶2A干扰核因子κB p65的二聚体形成和核转移[J]. 微生物与感染, 2014, 9(3): 147-151.
SHENG Lin-Jun, SONG Qin-Qin, LU Ming-Zhi, SONG Juan, SUN Peng, WANG Bao-Dong, CHI Miao-Miao, HAN Jun. Coxsackievirus B3 2A protease blocks nuclear translocation of nuclear factor κB (NF-κB) and inhibits dimerization of NF-κB. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(3): 147-151.