Isolation and identification of Mycobacterium from cows with delayed type hypersensitivity response to bovine purified protein derivative
WANG Quzhi1, JIANG Yuan2, SHEN Sufang1, SHEN Xin2, ZHAO Hongjin1, TANG Wenhong1, XU Feng1, YANG Xianchao1, LIU Peihong1
1. Shanghai Animal Disease Control Center, Shanghai 201103, China; 2. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
Abstract：To evaluate the specificity of single intradermal cervical tuberculin (SICT) of bovine purified protein derivative (PPD) in bovine tuberculosis detection, a total of 118 tissue specimens from 54 cows with positive reaction were collected and subjected to the detection of Mycobacterium. Acid-fast bacteria strains were isolated from 14 cows and Mycobacterium strains were isolated from 10 cows. Mycobacterium bovis (M. bovis) was isolated from one cow. Sixteen acid-fast bacteria strains were isolated from 118 tissue samples and 12 strains were identified as Mycobacterium. The isolation rate of Mycobacterium was 10.2% (12/118). One strain was identified as M. bovis and the others were nontuberculous Mycobacteria (NTM), such as M. avium and M. terrace. M. bovis accounted for 8.3% (1/12) and NTM accounted for 91.7% (11/12) of all Mycobacteria respectively. The results of pathogen detection indicated a low isolation rate of M. bovis by SICT method. To ensure the accuracy of SICT, it is necessary to use more than one method to verify the results. The culture and species identification by using rapid detection techniques are recommended to be introduced into the program for tuberculosis elimination, which could improve the specificity of tuberculosis detection.
王曲直1，江渊2，沈素芳1，沈鑫2，赵洪进1，唐文红1，徐峰1，杨显超1，刘佩红1. 牛型纯化蛋白衍生物皮内变态反应试验阳性牛中分枝杆菌的分离与鉴定[J]. 微生物与感染, 2016, 11(5): 268-272.
WANG Quzhi1, JIANG Yuan2, SHEN Sufang1, SHEN Xin2, ZHAO Hongjin1, TANG Wenhong1, XU Feng1, YANG Xianchao1, LIU Peihong. Isolation and identification of Mycobacterium from cows with delayed type hypersensitivity response to bovine purified protein derivative. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(5): 268-272.
2016, Vol. 11 
