
IL-22基因多态性与中国人群肺结核易感性的相关性研究
Gene polymorphisms of interleukin 22 and their interaction with susceptibility to tuberculosis in Chinese population
本研究旨在探讨白细胞介素22(interleukin 22,IL-22)基因多态性位点与肺结核易感性的关系。采用SNPscan™多重单核苷酸多态性(single nucleotide polymorphism,SNP)分型技术,对453例肺结核患者(结核病组)和373例与患者长期密切接触者〔包括109例潜伏结核感染(latent tuberculosis infection,LTBI)者、264例健康对照者〕的IL-22基因的4个SNP位点(rs1179249、rs2227491、rs17224704、rs2227478)多态性进行分析。结核病组、LTBI组和对照组中4个SNP位点的基因型分布经哈温平衡(Hardy-Weinberg equilibrium,HWE)检验均处于遗传平衡状态。结果显示,rs17224704位点存在AA、TA和TT基因型。>55岁人群中,此位点TA基因型在对照组中的分布显著高于结核病组(P=0.047 9,OR=0.365,95% CI=0.135~0.991);AA基因型在LTBI组中的分布显著低于结核病组(P=0.027 6);TA基因型在LTBI组中的分布显著高于结核病组(P=0.007 37,OR=0.213,95% CI=0.069~0.660)。对照组和LTBI组的等位基因T频率显著高于结核病组(P=0.026 9,OR=0.388,95% CI=0.167~0.897;P=0.025 0,OR=0.322,95% CI=0.119~0.867)。IL-22基因rs17224704的多态性可能与中国重庆地区汉族人群肺结核易感性显著相关,其等位基因T可能是肺结核的保护基因。
To investigate a potential impact of interleukin 22 (IL-22) gene polymorphisms on susceptibility to pulmonary tuberculosis (PTB) in a Chinese population, a case-control study was conducted in PTB cases (n=453), latent tuberculosis infection (LTBI) cases (n=109) and healthy controls (HCs) (n=264). Genetic polymorphisms of rs1179249, rs2227491, rs17224704, rs2227478 were analyzed by using SNPscan™ technique. The genetic frequencies of four single nucleotide polymorphisms (SNPs) met Hardy-Weinberg equilibrium. In the population older than 55, the frequency of genotype TA of rs17224704 SNP was significantly higher in healthy control group than that in PTB group (P=0.047 9,OR=0.365,95% CI=0.135-0.991). The frequency of genotype AA of rs17224704 SNP was significantly lower in LTBI group than that in PTB group (P=0.027 6). The frequency of genotype TA of rs17224704 SNP was significantly higher in LTBI group than that in PTB group (P=0.007 37, OR=0.213,95% CI=0.069-0.660). The frequency of allele T of rs17224704 SNP was significantly higher in healthy control group and LTBI group than that in PTB group (P=0.026 9, OR=0.388, 95% CI=0.167-0.897; P=0.025 0, OR=0.322, 95% CI=0.119-0.867). The results suggest that allele T of rs17224704 gene in Chinese Han population from Chongqing is probably associated with protection against tuberculosis.
结核分枝杆菌 / 结核病 / 易感性 / 白细胞介素22 / 单核苷酸多态性 / 潜伏结核感染
Mycobacterium tuberculosis;Tuberculosis / Susceptibility;Interleukin 22;Single nucleotide polymorphism / Latent tuberculosis infection
国家自然科学基金(81401711)
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