Abstract：This study aims to evaluate the immunological effects of mycobacterial protein subunit vaccine of Ag85a-interferon γ (Ag85a-IFN-γ). Firstly the plasmid pET28a-Ag85a-IFN-γ with the open reading frame of mature encoding sequence of Ag85A and IFN-γ was constructed, then expressed in Escherichia coli (E. coli) (DE3) BL21 cells. C57BL/6 mice were immunized by Ag85a-IFN-γ three times by subcutaneous injection biweekly. Two weeks after the last immunization, lymphocytes from spleens and sera were separated and detected for assessing the immunological effects. Serum antibodies against Ag85a (IgG, IgG1, and IgG2c) were detected by enzyme-linked immunosorbent assay (ELISA) and spleen lymphocytes subsets were analyzed by flow cytometry. The results demonstrated that the titers of antibodies (IgG, IgG1, and IgG2c) against the fusion protein Ag85a-IFN-γ in mice were higher than those in single antigen vaccinated mice and induced a T helper 1 (Th1) cell-mediate immunity response based on the ratio of IgG2c/ IgG1. The ratio of CD8+/CD4+ in Ag85a-IFN-γ vaccinated group was the highest among all groups. The results indicate that the fusion protein Ag85a-IFN-γ would be a promising subunit vaccine candidate for tuberculosis prevention.
王爽; 杨恩卓; 王洪海; 沈洪波. 新型抗结核蛋白亚单位疫苗Ag85A-γ干扰素的免疫效果评价[J]. 微生物与感染
, 2012, 7(3): 164-169.
WANG Shuang; YANG En-Zhuo; WANG Hong-Hai; SHEN Hong-Bo. Evaluation of the immunological effects of Ag85A-IFN-γ fusion protein on C57BL/6 mouse. Journal of Microbes and Infections, 2012, 7(3): 164-169.
