Efficacy and stability of maraviroc gel as a microbicide candidate in vitro
LI Liang-Fen1, BEN Yin-Yin1, LI Liang-Zhu1, ZHANG Xiao-Yan1,2
1. Shanghai Public Health Clinical Center Affiliated to Fudan University，Institutes of Biomedical Sciences，Fudan University，Shanghai 201508, China; 2. Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Basic Medical Sciences, Fudan University，Shanghai 200433, China
Abstract：To study the efficacy and stability of maraviroc gel in vitro, the cytotoxicity of maraviroc in phosphate buffered saline (PBS) or in 0.015% hydroxyethyl cellulose (HEC) were evaluated in TZM-bl and Jurkat-T cells. Meanwhile, two subtypes of human immunodeficiency virus (HIV) pseudovirus (SVPB16 and SVPC12) were used to evaluate the anti-HIV activity in vitro, the half maximal inhibitory concentration (IC50) or 90% maximal inhibitory concentration (IC90) of maraviroc gel were assessed. Furthermore, maraviroc was dissolved in 1.5% HEC with a final concentration of 6 mmol/L, and then maraviroc gel was stored at 4 ℃, room temperature, 30 ℃ and 40 ℃, respectively, in 75% relative humidity for 1 to 8 weeks. The anti-HIV activity was tested weekly. The data showed that maraviroc gel had nearly same cytotoxicity as maraviroc in PBS. HEC did not weaken the ant-HIV activity of maraviroc. Inversely, HEC might slightly enhance the anti-HIV efficacy of maraviroc. Maraviroc gel was stable for at least 8 weeks even at 40 ℃. It is concluded that maraviroc gel has good efficacy and stability as a microbicide candidate.
李良峰1，贲银银1，李亮助1，张晓燕1,2. 马纳维诺凝胶作为候选杀微生物剂的生物学活性和稳定性体外评价[J]. 微生物与感染, 2014, 9(2): 102-106.
LI Liang-Fen1, BEN Yin-Yin1, LI Liang-Zhu1, ZHANG Xiao-Yan1,2. Efficacy and stability of maraviroc gel as a microbicide candidate in vitro. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(2): 102-106.
