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微生物与感染  2019, Vol. 14 Issue (1): 23-29    DOI: 10.3969/j.issn.1673-6184.2019.01.004
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影响金黄色葡萄球菌杀白细胞素ED转录表达的培养条件
杨涵1,黄凯峰1,徐溯2,赵焕强1,徐晓刚2,胡付品2,何春燕1,龚芳3,刘庆中1
1. 上海交通大学附属第一人民医院检验医学中心,上海 200080; 2. 复旦大学附属华山医院抗生素研究所,上海 200040; 3. 南通大学附属南通第三医院检验科,江苏 南通 226000
Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus
YANG Han1, HUANG Kaifeng1, XU Su2, ZHAO Huanqiang1, XU Xiaogang2, HU Fuping2, HE Chunyan1, Gong Fang3, LIU Qingzhong1
1. Department of Clinical Laboratory, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China; 2. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China; 3. Department of Clinical Laboratory, The Third Hospital Affiliated to Nantong University, Nantong 226000, Jiangsu Province, China
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摘要: 为探讨影响金黄色葡萄球菌杀白细胞素ED(leukocidin ED,LukED)转录表达的最佳培养条件,采用定量反转录-聚合酶链反应(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)检测金黄色葡萄球菌Newman菌株lukE在TSB、BHI、YCP、MHB和LB培养基中不同生长阶段(对数生长早期、中期、晚期和平台期)的表达情况。以表达量较低的培养基为基础,分别加入表达量最高的培养基的不同成分,分析促进lukED表达的物质。结果显示,lukE mRNA水平在YCP培养基中生长至对数晚期时表达最高;YCP组分酵母浸出物、酪蛋白氨基酸及丙酮酸钠均能显著促进lukE转录表达,前两者效果更好。

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作者相关文章
杨涵1
黄凯峰1
徐溯2
赵焕强1
徐晓刚2
胡付品2
何春燕1
龚芳3
刘庆中1
关键词 金黄色葡萄球菌杀白细胞素EDmRNA水平生长阶段培养基    
Abstract:This study aimed to explore the optimal culture conditions for transcriptional expression of leukocidin ED (lukED) of Staphylococcus aureus (S. aureus). The transcription level of lukE in S. aureus strain Newman was detected at different growth phases (early-exponential, middle-exponential, late-exponential and stationary phases) in TSB, BHI, YCP, MHB and LB media by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Based on the medium mediating the lowest transcription level of lukE, the different compositions of medium producing the highest mRNA level of lukE was added to discover the substances promoting lukED expression. The findings showed that lukE mRNA level was highest at the late-exponential phase in YCP medium, in which yeast extract, casamino acids and sodium pyruvate could all strikingly increase the expression of lukE, with the first two being better.

Key wordsStaphylococcus aureus    Leukocidin ED    mRNA level    Growth phase    Medium
基金资助:国家自然科学基金(81772247,81371872)

通讯作者: 刘庆中   
引用本文:   
杨涵1,黄凯峰1,徐溯2,赵焕强1,徐晓刚2,胡付品2,何春燕1,龚芳3,刘庆中1. 影响金黄色葡萄球菌杀白细胞素ED转录表达的培养条件[J]. 微生物与感染, 2019, 14(1): 23-29.
YANG Han1, HUANG Kaifeng1, XU Su2, ZHAO Huanqiang1, XU Xiaogang2, HU Fuping2, HE Chunyan1, Gong Fang3, LIU Qingzhong1. Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus. JOURNAL OF MICROBES AND INFECTIONS, 2019, 14(1): 23-29.
链接本文:  
http://jmi.fudan.edu.cn/CN/10.3969/j.issn.1673-6184.2019.01.004     或     http://jmi.fudan.edu.cn/CN/Y2019/V14/I1/23

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