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Activation analysis of CD4+ and CD8+T cells in peripheral blood and pleural effusion in patients with tuberculous pleurisy |
ZHU Jie-Hua1, LUO Jun-Min1,YE Jun2,QIN Huan1 , JIANG Juan3, SHAO Yun-Hao4 |
1. Department of Immunology, Immunology Innovation Base of Postgraduate Education of Guizhou Province, Zunyi Medical College, Zunyi 563003, China;2. Clinical Laboratory, The Second Affiliated Hospital of Guiyang Medical College, Kaili 550003, China;3. Department of Medical Laboratory, Zunyi Medical College, Zunyi 563003, China;4. Department of Medical Cosmetology, Zunyi Medical College, Zunyi 563003, China |
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Abstract The pathogenesis of tuberculous pleurisy remains unclear. Evidences have shown that immuno-imbalance is an important risk factor for this condition. The present paper aimed to profile CD4 + and CD8 + T cells in patients with tuberculous pleurisy. A total of 30 cases were selected and their peripheral blood and pleural fluid were collected. The mononuclear cells were isolated. The CD4 + cells, CD8 + T cells, activation surface markers of human leukocyte antigen (HLA)-DR, and granzyme B level in CD8+ T cells were detected by flow cytometry. The results showed that the proportions of CD3 + , CD4 + and CD8 + T cells in peripheral blood samples collected from the patients with tuberculous pleurisy were lower than those in the control group. The proportions of CD3 + and CD4 + T cells in patients' pleural effusion were higher than those in peripheral blood, but the proportion of CD8 + T cells had no significant difference. The levels of HLA-DR on CD4 + and CD8 + T cells in peripheral blood in patients with tuberculous pleurisy were higher than those in the control group. And the level of CD8+ granzyme B+ T cells in patients with tuberculous pleurisy was lower than that in the control group. In patients , the levels of CD4 + HLA + , CD8 + HLA + and CD8+ granzyme B+ T cells in pleural effusion were lower than those in peripheral blood. In patients' peripheral blood there was a positive correlation between CD8 + T cells and granzyme B level. The results suggested that the immune response between peripheral blood and pleural fluid in patients with tuberculous pleurisy were different. Immuno-imbalance may play a critical role in the development/prognosis of the disease.
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Received: 28 December 2012
Published: 25 June 2013
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Corresponding Authors:
LUO Jun-Min
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