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Abstract Objective To investigate the differential expression of miRNA in pleural fluid and serum exosomes from patients with tuberculous pleurisy, which is to provide a basis for diagnosis and differential diagnosis of tuberculous pleurisy. Methods 20 patients' serum and pleural fluid who with tuberculous pleurisy (experiment group) and that who have pneumonia with inflammatory pleural effusion (control group 2) , 20 healthy persons' serum (Control Group 1)wer all collected.After isolated exosomes and extrated the total RNA , we analyzed the miRNA expression level with real-time quantitative PCR. Results Compared with control group 1, the level of miRNA expression in serum exosomes from control group2 was increased in different degree, among them the Let-7a-5p, Let-7c-5p, Let-7i-5p and miRNA-126-3p increased significantly, the expression level of miRNA in the serum exosomes from experimental group was higher than that of control group2, especially let-7a-5p, Let-7d-5p, Let-7e-5p, miRNA-126-3p, Let-7g-5p and let-7c-5p (p < 0.05).Compared with control group 2, the expression levels of miRNA in pleural effusion exosomes from the experimental group were higher, and let-7f-5p, Let-7g-5p, let-7d-3p were higher than those in the control group2.The expression of miRNA in pleural effusion and serum exosomes was also significantly different in the experimental group.Conclusion The experession of mirnas in pleural fluid and serum exosomes from patients with tuberculous pleurisy were different from general pneumonia with pleural effusion , the hsm-let-7 family members might be used as a good differential diagnostic index between tuberculous pleural effusion and common inflammatory pleural effusion.
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Received: 24 April 2023
Published: 25 August 2023
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