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| The value of miRNA hsm-let-7 family from exosomes in differential diagnosis of pleural effusion |
| DONG Zeling1, DENG Fenglin1, ZHU Jiehua1, WANG Juan2, DU Wensheng1 |
| 1. Department of Medical Laboratory, Affiliated Hospital of Zunyi Medical University, Zunyi 563099, Guizhou Province, China; 2. Department of Neurology, First People’s Hospital of Zunyi, Zunyi 563003, Guizhou Province, China |
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Abstract The purpose of this study is to investigate the differential expression of miRNA in pleural fluid and serum exosomes from patients with tuberculous pleurisy, which is to provide a basis for diagnosis and differential diagnosis of tuberculous pleurisy. Serum and pleural fluid specimens from 20 patients who had tuberculous pleurisy (experiment group) and 20 patients who had pneumonia with inflammatory pleural effusion (control group 2) were collected. 20 healthy persons’ serum (control group 1) were collected, too. The exosomes were separated and total RNA were extracted for expression level analysis of miRNA with real-time quantitative polymerase chain reaction (PCR) technology. The results showed that compared with control group 1, the level of miRNA expression in serum exosomes from control group 2 was increased in different degree, among them the let-7a-5p, let-7c-5p, let-7i-5p and miRNA-126-3p increased significantly. The expression level of miRNA in the serum exosomes from experimental group was higher than that of control group 2, especially let-7a-5p, let-7d-5p, let-7e-5p, miRNA-126-3p, let-7g-5p and let-7c-5p (P<0.05). Compared with control group 2, the expression levels of miRNA in pleural effusion exosomes from the experimental group were higher, and let-7f-5p, let-7g-5p, let-7d-3p were obviously higher than those in the control group 2. The expressions of miRNA in pleural effusion and serum exosomes were also significantly different in the experimental group. So it was concluded that the expressions of miRNA in pleural fluid and serum exosomes from patients with tuberculous pleurisy were different from general pneumonia with pleural effusion. The hsm-let-7 family members might be used as a good differential diagnostic index between tuberculous pleural effusion and common inflammatory pleural effusion.
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Received: 24 April 2023
Published: 25 August 2023
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Corresponding Authors:
DU Wensheng
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| [1] |
HU Qiankun, WANG Qianqian, LI Qiang, HUANG Chenlu, XU Wei, ZHANG Yi, LI Xinyan, CHEN Liang, HUANG Yuxian. Baseline serum exosomal miR-155-5p combined with HBV DNA can predict HBeAg seroconversion in chronic hepatitis B patients receiving peginterferon[J]. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(6): 385-392. |
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2023, Vol. 18 
