HBsAg interferes with the TLR2 and TLR4 signaling pathway

LIU Hua; CHEN Zhiao; CHENG Yu-ming; YUAN Zheng-hong

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Journal of Microbes and Infections ›› 2008, Vol. 3 ›› Issue (2) : 84-89.

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HBsAg interferes with the TLR2 and TLR4 signaling pathway

LIU Hua , CHEN Zhiao , CHENG Yu-ming, YUAN Zheng-hong

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Abstract

Objective　To investigate the role of Hepatitis B surface antigen (HBsAg) in the immune escape of innate immunity by Hepatitis B virus (HBV).　Methods After treated with PMA, THP-1 was differentiated into macrophage- like cells. Macrophage- like cells were treated further with LPS and the pam3csk4 in the presence or absence of HBsAg. Cytokine IL-10 , IL-12 protein, IL-10, IL-12 mRNA levels , NF-κB p65 protein nuclear translocation as well as IκB-α degradation and ERK protein phosphorylation were detected to monitor the activation of the TLR signaling pathway. Results LPS and the pam3csk4 induced production of cytokine IL-10 , IL-12 protein, NF-κB p65 protein nuclear translocation as well as IκB-α degradation and ERK protein phosphorylation were inhibited by HBsAg in a dose-dependent manner. Conclusion HBsAg inhibit the activation of TLR2 and TLR4 signaling pathway.

Key words

Hepatitis B virus surface antigen / Toll-like receptor / Macrophage-like cell / THP-1

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LIU Hua , CHEN Zhiao , CHENG Yu-ming, YUAN Zheng-hong. HBsAg interferes with the TLR2 and TLR4 signaling pathway[J]. Journal of Microbes and Infections. 2008, 3(2): 84-89