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| Distribution of N-glycosidase genes in clinical isolates of Elizabethkingia meningoseptica |
| LI Meng, SUN Gui-Qin, CHEN Jing, LI Tian-Sheng, WANG Lei, Celimuge, CHEN Li |
| Key Laboratory of Medical Molecular Virology of Ministries of Health and Education, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China |
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Abstract The present paper aims to investigate the distribution of two peptide: N-glycanase (PNGase) genes in Elizabethkingia meningosepticum (EM). The genomic DNAs of 65 clinical strains isolated from 3 hospitals in Zhejiang Province were extracted and subjected to polymerase chain reaction (PCR) for 16S rRNA, PNGase F and PNGase F-Ⅱ genes respectively. The PCR products were sequenced and compared to the known sequences in National Center of Biotechnology Information (NCBI). The results showed that the positive rate of PNGase gene was 98.5% and the PNGase F/PNGase F-Ⅱ gene coexistence rate was 60.0%. The positive rates of PNGase F gene and PNGase F-Ⅱ gene were 63.1% and 95.4%, respectively (matching χ2 test, P<0.01). The results indicate that the developed PCR can be used to detect PNGase gene in EM strains and PNGase activity may be crucial for the survival.
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Received: 18 June 2014
Published: 25 December 2014
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Corresponding Authors:
CHEN Li
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