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| Immunological evaluation of BALB/c mice as an animal model for herpes simplex virus 1 infection |
| ZHANG Xiaolong1,*, TANG Beibei1,*, HE Yufeng1, DUAN Yongzhong1, WANG Lichun1, ZHANG Zhixiao1, ZHOU Jumin2, LI Qihan1 |
| 1. Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China; 2. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China |
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Abstract To investigate the immunological responses of BALB/c mice to herpes simplex virus type 1 (HSV-1) infection, the following factors were followed: strains, infect routes and mouse ages, the dendritic cells (DCs) and HSV-1 specific T cell rate and function in peripheral blood mononuclear cells (PBMCs), serum neutralizing antibody titer, and CD8＋ T cell infiltration in nervous tissues in HSV-1-infected BALB/c mice. The results showed that 3-week- and 6-week-old BALB/c mice challenged with HSV-1 Mckrae and 17＋ strains through corneal and nasal routes led to an increased rate of DCs in PBMCs, and conferred DC capacity to stimulate HSV-1 specific T cells in vivo. At 35 d post infection, low level interferon γ (IFN-γ)＋ T cells and no interleukin 4 (IL4)＋ T cells could be detected in PBMCs, and low level neutralizing antibody was present or absent in serum. Ninety days after BALB/c mice challenged with HSV-1 through subcutaneous and foot pad injection, CD8＋ T cells could be detected in mouse trigeminal ganglia in different groups. However, HSV-1 specific IFN-γ＋ T cell and IL4＋ T cell rates showed divergence between different virus strains and mouse ages. Foot pad injection of BALB/c mice with HSV-1 could elicit higher neutralizing antibody titer compared with subcutaneous injection. This research demonstrated that, infection of different age BALB/c mice with different HSV-1 strains through different routes can stimulate comparable DC maturation and antigen presentation.
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Received: 02 March 2017
Published: 25 August 2017
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Corresponding Authors:
LI Qihan
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2017, Vol. 12 
