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| Impact of environmental stresses on transcriptional regulation of Rcs in Shigella flexneri |
| YE Li-Na, CAI Xia, LIN Zhiwei, LV Zhihui, WANG Xiaofei, SHANG Yongpeng, QU Di |
| Department of Medical Microbiology and Parasitology，Key Laboratory of Medical Molecular Virology of MOE & MOH，School of Basic Medical Sciences，Fudan University，Shanghai 200032，China |
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Abstract The regulator of colanic acid capsule synthesis (Rcs) is a complicated two-component signal transduction system. Although details of Rcs in most Enterobacteria are known, its regulation in Shigella has not been reported yet. This study aims to explore the influences of environmental stresses on Rcs transcription in Shigella. Based on biological informatics analysis of RcsBDC in Shigella flexneri 2a 301, the transcriptional levels of rcsB, rcsD, rcsC at different growth stages were analyzed by real-time polymerase chain reaction (PCR), and the changes in the transcriptional levels of the three genes in acid and osmotic pressure conditions were examined. The transcriptional levels of the three genes were higher in 5－6 h (logarithmic phase), and lower in 8－10 h (stationary phase). The transcriptional levels of rcsB and rcsD in acid and osmotic pressure conditions were higher than in normal condition, suggesting environmental stimuli promote the transcriptional levels of rcsB and rcsD in Shigella flexneri during stationary phase.
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Received: 04 April 2018
Published: 01 January 2018
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Corresponding Authors:
QU Di
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