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Enhancement of the mucosal immune responses and prophylactic effect of a novel coxsackievirus B3-specific mucosal DNA vaccine by co-administration of lymphotactin |
Yue Yan; Hu Lin-Kun; Xu Wei; Xiong Si-Dong |
Institute for Immunobiology, Shanghai Medical college, Fudan University, Shanghai 200032, China |
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Abstract To improve the mucosal immune responses and protection against coxsackievirus B3 (CVB3), on the basis of our previously prepared chitosan-pVP1 mucosal vaccine, plasmid encoding lymphotactin (LTN), a C family chemokine, was introduced. chi-(pVP1+pLTN) were prepared by conjugating chitosan with the mixture of 50 μg pVP1 and pLTN. Male BALB/c mice were immunized 4 times biweekly. Two weeks after the final immunization, the level of CVB3-specific serum IgG, fecal IgA and mucosal CTL activity were quantified. Meanwhile, mice were challenged with 3 LD50s, serum creatine kinase (CK) activity and histopathological changes in heart tissue were measured. Compared with control groups, immunization with chi-(pVP1+pLTN) significantly increased the levels of CVB3-specific serum IgG and fecal IgA, and enhanced the mucosal CTL activity. The incidence of myocarditis in mice receiving chi-(pVP1+pLTN) was 16.7%, significantly lower than 33.3% in mice receiving chi-(pVP1+pcDNA3.1). Histopathological analysis showed that fewer inflammatory cells under the epicardium in the hearts of co-immunized mice compared with that of control groups. In conclusion, intranasal co-administration of LTN plasmid via chitosan nanoparticle could enhance CVB3 specific mucosal immune responses and provide more efficient protection against CVB3-induced myocarditis.
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Received: 04 March 2010
Published: 25 June 2010
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Corresponding Authors:
Xiong Si-Dong; Xu Wei
E-mail: sdxiong@shmu.edu.cn; wx2362@hotmail.com
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Cite this article: |
Yue Yan,Hu Lin-Kun,Xu Wei等. Enhancement of the mucosal immune responses and prophylactic effect of a novel coxsackievirus B3-specific mucosal DNA vaccine by co-administration of lymphotactin[J]. Journal of Microbes and Infections, 2010, 5(2): 77-83.
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URL: |
http://jmi.fudan.edu.cn/EN/ OR http://jmi.fudan.edu.cn/EN/Y2010/V5/I2/77 |
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