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JOURNAL OF MICROBES AND INFECTIONS  2019, Vol. 14 Issue (4): 199-208    DOI: 10.3969/j.issn.1673-6184.2019.04.002
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Construction of Flag-GPI carrying recombinant hepatitis B virus vector
HU Kongying1, GU Chenjian1, WU Minle2, TAO Shuai1, LIU Nannan1, LIU Jing1, XIE Youhua1
1. Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University, Shanghai 201399, China

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Abstract  Hepatitis B virus (HBV) is a small hepatophilic DNA virus that can specifically infect human hepatic parenchymal cells. The susceptibility of HBV can be attributed to cell surface receptor(s), intracellular proteins and other factors. A simple and clear method to assay the susceptibility of cells for HBV remains a challenge. Here, we constructed the recombinant HBV vectors 5c3c-CD59-Flag-GPI and 5c3cT-Flag-GPI based on the original HBV 5c3c vector and glycosylphosphatidylinositol (GPI) anchor. After transfection into Huh7 cells, Flag tags can be anchored on the cell membrane, and sorted by flow cytometry using Flag antibody. With the replenishment of HBV envelope protein, 5c3cT-Flag-GPI can form complete recombinant HBV particles. This study laid a foundation for optimization of packaging efficiency of recombinant HBV, which would provide a tool for HBV susceptibility research.
Key wordsHepatitis B virus      5c3c      Glycosylphosphatidylinositol      Recombinant hepatitis B virus vector     
Received: 23 April 2019      Published: 25 August 2019
Corresponding Authors: XIE Youhua   
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HU Kongying1
GU Chenjian1
WU Minle2
TAO Shuai1
LIU Nannan1
LIU Jing1
XIE Youhua1
Cite this article:   
HU Kongying1,GU Chenjian1,WU Minle2等. Construction of Flag-GPI carrying recombinant hepatitis B virus vector[J]. JOURNAL OF MICROBES AND INFECTIONS, 2019, 14(4): 199-208.
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http://jmi.fudan.edu.cn/EN/10.3969/j.issn.1673-6184.2019.04.002     OR     http://jmi.fudan.edu.cn/EN/Y2019/V14/I4/199
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