In order to investigate the distribution of serotypes and virulence genes of hypervirulent Klebsiella pneumoniae (HVKP) and to explore molecular markers, this study collected Klebsiella pneumoniae strains and divided into invasive syndrome KP group and bloodstream infection KP group.The results showed that the positive rate of serotype K1 in the invasive syndrome KP group was 60%, which was significantly higher than that in the bloodstream infection KP group. The rate of uge was the highest, followed by fimH. Overall, the positive rate of invasive syndrome KP virulence gene distribution was higher than that of bloodstream infection KP, especially rmpA2,magA, fimH,aero,iutA,kfuBC.According to the Yoden index, the diagnostic efficacy of these molecular markers was ranked from high to low: iutA>kfuBC> magA(K1)>aero>fimH>rmpA2. Multivariate logistic regression analysis showed that rmpA2, magA, fimH, aero, iutA and kfuBC could be used as molecular markers of HVKP, especially iutA, but lack 100% specific molecular markers, and further exploration is needed.