Abstract The incidence of respiratory infections is high, early identification of infectious pathogens is the key to improve the cure rate, reduce the mortality.At present, pathogen culture is still the main method of clinical etiology diagnosis, but the low sensitivity and long time are not conducive to early diagnosis and treatment. Metagenomics next-generation sequencing covers the advantages of wide, rapid, unbiased and no specific amplification of pathogens, and has high clinical application value in the identification of rare, mixed infection, immunosuppressive patient infection and the diagnosis of pathogens that are difficult to be detected by conventional detection methods. However, some of them have low specificity, lack of recognized interpretation standards, unclear relationship between sequencing results and treatment, difficulty in testing drug-resistant genes, and high price. At present, in clinical application, metagenomics next-generation sequencing and traditional microbial detection technology have a complementary effect, and the combination of the two can improve the clinical diagnosis efficiency.
Received: 26 October 2020
Published: 01 January 2021