The application of variable number of tandemrepeats in the microevolution study of Mycobacterium tuberculosis strain of Beijing genotype in Chongming Island, Shanghai
QIAO Ke1,*; WANG Hui1,*; YANG Chong-Guang1; LUO Tao1; MEI Jian2; GAO Qian1
1. Key Laboratory of Medical Molecular Virology of Ministry of Education and Health, Shanghai Medical School, Fudan University, Shanghai 200032, China; 2. Department of Tuberculosis Disease Control, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
Abstract：To find the variable number of tandemrepeat ( VNTR) locus combinations that adapt to study the microevolution of Mycobacterium tuberculosis ( M. tuberculosis ) strain of Beijing genotype and the molecular markers to distinguish diff erent sublineages , VNTR and single nucleotide polymorphism( SNP) genotyping were usedto genotype 135 strains fromculture-positive sputumof tuberculosis patients ( collected and preserved by Shanghai Municipal Center f or Disease Control and Prevention) in Chongming Island , Shanghai . Based on the data, the mini mumspanning tree ( MST) was constructed . The results showed that the genotyping inf ormation of 16-locus VNTR best matched the SNP genotyping data, indicating that it was applicable in the microevolution study of M. tuberculosis strain of Beijing genotype in Chongming Island ,
Shanghai . SNP hapl otypes were mainly concentrated in ST10 ( 42. 2%) , ST19 ( 30. 4%) , and ST22 (14.1 %) . Four VNTRloci ( Mtub21 , QUB26 , MI RU16 and QUB4156) may be the molecular markers to distinguish different sublineages of Beijing genotype strains .
乔可; 王辉; 杨崇广; 罗涛; 梅建; 高谦. 可变数目串联重复序列在上海崇明岛地区结核分枝杆菌北京基因型菌株微进化研究中的应用[J]. 微生物与感染
, 2010, 5(4): 208-213.
QIAO Ke1,*; WANG Hui1,*; YANG Chong-Guang1; LUO Tao1; MEI Jian2; GAO Qian1. The application of variable number of tandemrepeats in the microevolution study of Mycobacterium tuberculosis strain of Beijing genotype in Chongming Island, Shanghai. Journal of Microbes and Infections, 2010, 5(4): 208-213.
