Abstract：African swine fever virus (ASFV) poses a serious threat to animal health, food safety, and even the global economy. To date, there is still no ASFV vaccine or therapeutic available. For biosafety reasons, most researches on ASFV must be conducted in biosafety level 3 (BSL-3) laboratories. This is the main obstacle for the study on ASFV, including its pathogenesis and escape mechanism, and the development of ASFV vaccines and therapeutics. In this study, we selected some ASFV envelope proteins that are reported to participate in the process of viral entry into the host cell and used the vesicular stomatitis virus (VSV) pseudovirus packaging system to produce several ASFV pseudoviruses. We found that one of these ASFV pseudoviruses could effectively infect some ASFV-susceptible cells, and thus established an ASFV pseudovirus infection cell model, which could be used in laboratories with a lower biosecurity level. As the pseudovirus contains a Renilla luciferase reporter gene, this ASFV pseudovirus could be used for screening of ASFV entry inhibitors and neutralizing antibodies, thus providing a safe and convenient model for research and development of antiviral drugs against ASFV infection.
唐嘉怡，蔡艳星，王欣玲，姜世勃，陆路. 一种非洲猪瘟病毒假病毒细胞感染模型的建立及应用[J]. 微生物与感染, 2021, 16(5): 304-312.
TANG Jiayi, CAI Yanxing, Wang Xinling, JIANG Shibo, LU Lu. The establishment and application of an ASFV pseudovirus infection cell model. JOURNAL OF MICROBES AND INFECTIONS, 2021, 16(5): 304-312.
2021, Vol. 16 
