The value of miRNA hsm-let-7 family from exosomes in differential diagnosis of pleural effusion
DONG Zeling1, DENG Fenglin1, ZHU Jiehua1, WANG Juan2, DU Wensheng1
1. Department of Medical Laboratory, Affiliated Hospital of Zunyi Medical University, Zunyi 563099, Guizhou Province, China; 2. Department of Neurology, First People’s Hospital of Zunyi, Zunyi 563003, Guizhou Province, China
Abstract：The purpose of this study is to investigate the differential expression of miRNA in pleural fluid and serum exosomes from patients with tuberculous pleurisy, which is to provide a basis for diagnosis and differential diagnosis of tuberculous pleurisy. Serum and pleural fluid specimens from 20 patients who had tuberculous pleurisy (experiment group) and 20 patients who had pneumonia with inflammatory pleural effusion (control group 2) were collected. 20 healthy persons’ serum (control group 1) were collected, too. The exosomes were separated and total RNA were extracted for expression level analysis of miRNA with real-time quantitative polymerase chain reaction (PCR) technology. The results showed that compared with control group 1, the level of miRNA expression in serum exosomes from control group 2 was increased in different degree, among them the let-7a-5p, let-7c-5p, let-7i-5p and miRNA-126-3p increased significantly. The expression level of miRNA in the serum exosomes from experimental group was higher than that of control group 2, especially let-7a-5p, let-7d-5p, let-7e-5p, miRNA-126-3p, let-7g-5p and let-7c-5p (P<0.05). Compared with control group 2, the expression levels of miRNA in pleural effusion exosomes from the experimental group were higher, and let-7f-5p, let-7g-5p, let-7d-3p were obviously higher than those in the control group 2. The expressions of miRNA in pleural effusion and serum exosomes were also significantly different in the experimental group. So it was concluded that the expressions of miRNA in pleural fluid and serum exosomes from patients with tuberculous pleurisy were different from general pneumonia with pleural effusion. The hsm-let-7 family members might be used as a good differential diagnostic index between tuberculous pleural effusion and common inflammatory pleural effusion.
董泽令1，邓凤琳1，朱杰华1，王娟2，杜文胜1. 外泌体miRNA hsm-let-7家族在胸水病因鉴别诊断中的价值初探[J]. 微生物与感染, 2023, 18(4): 211-218.
DONG Zeling1, DENG Fenglin1, ZHU Jiehua1, WANG Juan2, DU Wensheng1. The value of miRNA hsm-let-7 family from exosomes in differential diagnosis of pleural effusion. JOURNAL OF MICROBES AND INFECTIONS, 2023, 18(4): 211-218.
2023, Vol. 18 
