本研究旨在评估大连医科大学医学微生物学课程线上教学效果，及时发现和总结线上教学中存在的问题，为教学课程改革提供依据。使用大连医科大学网络题库与考试评价系统对2021年参加医学微生物学课程线上学习和考试的641名学生的试卷进行分析，分析内容包括试卷各章节的正确率、难度系数、信度和区分度。结果显示，本次考试成绩呈正态分布，各章节正确率为70%～90%，考试平均分72.28，及格率85%，优秀率4%。试卷总体质量良好，难度略偏易，难度系数0.72。信度和区分度好，区分度0.29，信度为0.9。2021年线上教学与2019年线下教学的学生期末试卷成绩相比无显著性差异(P＞0.05)。本研究证实本次线上考试试卷总体质量良好，能够客观体现教学质量和学生学业水平，线上教学效果基本满意。本研究还比较了试卷中各题型的难度系数和区分度，为今后制定科学合理的线上考核试卷提供依据和参考。

The purpose of this study is to evaluate the effect of online teaching of medical microbiology course in Dalian Medical University, find and summarize the problems in online teaching in time, and provide a basis for teaching curriculum reform. The examination paper of 641 students who participated in the online teaching and examination of medical microbiology course in 2021 was analyzed by using the network question bank and examination evaluation system of Dalian Medical University, including the accuracy rate of each chapter of the paper, difficulty coefficient, reliability and discrimination. The results showed that the score of this exam was normal distribution, the accuracy of each chapter was between 70% and 90%, the average score of the exam was 72.28, the pass rate was 85%, and the excellent rate was 4%. The overall quality of the examination paper was good.The difficulty of the examination paper was slightly easier, and the difficulty coefficient was 0.72. The discrimination was 0.29, the reliability was 0.9. There was no significant difference in the final exam scores between online teaching students in 2021 and offline teaching students in 2019 (P>0.05). This study confirms that the overall quality of the online examination paper is good, which can objectively reflect the teaching quality and students’ academic level. The online teaching effect is basically satisfied. This study also compares the difficulty coefficient and differentiation of each question type in the test paper to provide the basis and reference for the development of scientific and reasonable online examination papers in the future.