摘要
目的 调查上海市生活饮用水中非结核分枝杆菌的状况及主要菌种分布。方法 应用过滤法收集细菌, 并在改良罗氏培养基上培养; 通过16S rRNA 测序鉴定菌种。结果 上海市生活饮用水中非结核分枝杆菌的检出率为16. 7% , 其中自来水厂原水、出厂水和居民生活饮用终端水的检出率分别为60% 、25% 和10. 3% 。分离鉴定的非结核分枝杆菌菌种为戈登分枝杆菌及偶发分枝杆菌, 分别占90% 及10% 。结论 在上海市居民生活饮用水系统中存在非结核分枝杆菌, 因此, 应当采取有效的控制方法以保护公众的健康。
Abstract
Objective To survey the distribution of nontuberculous mycobacteria in drinking water distribution systems in Shanghai. Methods Samples of tap water, source water, and treated water from water corporations were collected. The bacteria were collected through filtrating and culturing on Lowenstein-Jensen medium. The nontuberculous mycobacteria were identified by sequencing 16S rRNA. Results The positive rates of nontuberculous mycobacteria in source water, treated water, and tap water were 60% , 25% , and 10. 3% , respectively. Mycobacterium gordonae and Mycobacterium fortuitum were identified, of which Mycobacterium gordonae was the most frequently identified species. Conclusion Nontuberculous mycobacteria are widely distributed in drinking water distribution systems in Shanghai. Thus, effective control measures should be taken to protect the health of the people.
关键词
非结核分枝杆菌 /
分离
Key words
Drinking water distribution systems /
Nontuberculous mycobacteria /
Isolation
陈超1 ;徐鹏1 ;李静1 ;武洁1 ;高谦2 ;梅建1.
城市生活饮用水中非结核分枝杆菌调查[J]. 微生物与感染. 2008, 4(4): 215-218
CHEN Chao1;XU Peng1;LI Jing1;WU Jie1;GAO Qian2;MEI Jian1.
An investigation of the nontuberculous mycobacteria in drinking water distribution systems in Shanghai[J]. Journal of Microbes and Infections. 2008, 4(4): 215-218
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