目的 建立一种检测解脲脲原体生物群1、2的聚合酶链反应-毛细管电泳(PCR-CE)方法,研究解脲脲原体生物群与男性非淋菌性尿道炎(NGU)的关系。方法 合成解脲脲原体生物群1、2的通用引物,进行PCR,然后使用CE检测解脲脲原体生物群1、2的扩增产物。对该方法进行敏感性、特异性的评价。使用该方法检测不同男性人群尿样中解脲脲原体2个生物群。结果 PCR-CE法的敏感性为10拷贝/50μl。该方法仅特异扩增解脲脲原体生物群1、2。解脲脲原体生物群2在NGU组、非沙眼衣原体引起的NGU(NCNGU)组中的检出率高于对照组(P<0.05),解脲脲原体生物群1在NGU及NCNGU组中的检出率和对照组无差异(P>0.05)。结论 建立了一种敏感性高、特异性强、分辨率高的检测解脲脲原体2个生物群的PCR-CE方法。解脲脲原体生物群2与男性NGU有一定的关系。解脲脲原体生物群1不能引起男性NGU。

Objective To develop a polymerase chair reaction-capillary electrophoresis(PCR-CE)based method for the detection of biovars one and two of U.urealyticum and to determine whether these two biovars of U.urealyticum are associated with the nongonococcal urethritis(NGU)in the male patients.Methods Common primers for two biovars of U.urealyticum were synthesized and used for the PCR.Then PCR products obtained for two biovars of U.urealyticum were detected using capillary electrophoresis(CE).Two biovars of U.urealyticum were detected by the above method in the urine samples from the different male populations.The sensitivity and specificity of this PCR-CE based method was further examined.Results This PCR-CE method had a sensitivity of detection at 10 copies /50 μl.This method specifically amplified biovar 1 and biovar 2 of U.urealyticum.The prevalence of U.urealyticum biovar 2 in NGU group and nonchlamydial NGU(NCNGU)group was higher than that in the control group(P<0.05)but the prevalence of U.urealyticum biovar 1 in NGU group and NCNGU group did not differ significantly from that in the control group(P>0.05).Conclusion A PCR-CE based method for the detection of two biovars of U.urealyticum has been developed,which has good sensitivity and specificity.U.urealyticum biovar 2 is associated with NGU in the male.U.urealyticum biovar 1 can not cause NGU.