摘要
目的 建立马内菲青霉蛋白酶的体外诱导方法并进行活性测定。方法 制作酶诱导培养基, 在不同pH 条件下培养马内菲青霉, 在280 nm紫外光下分光光度法检测不同诱导时间的蛋白酶活性。结果 成功诱导并定量测定了马内菲青霉在不同pH条件下、不同诱导时间的体外蛋白酶产出量。pH 4. 0 时, 菌株在第6 天出现分泌高峰; pH 5. 6 时, 菌株在第3 天出现分泌高峰; 而pH 7. 2 时, 菌株几乎无蛋白酶产出。结论 建立了马内菲青霉蛋白酶产出的体外诱导和检测方法, 为进一步研究马内菲青霉体外蛋白酶的理化特性提供了途径。
Abstract
Objective To establish a method of inducing proteinase production of Penicillium marneffei in vitro and to measure its proteinase activity. Methods The B-6323 strain of P. marneffei was cultured in proteinase inducing medium under different pH conditions, and the production of proteinase over the course of several days under different pH conditions was measured using a spectrophotometric method ( 280 nm UV light) . Results Proteinase was induced and it’s activity was measured over the course of several days under different pH conditions. The strain had the highest proteinase activity on day 6 at pH 4. 0; the highest proteinase activity on day 3 at pH5. 6; and no proteinase activity at pH7. 2 on any day. Conclusion A method of inducing proteinase production of P. marneffei in vitro and measuring its activity was established, and may be amethod to be used for future research to understand the biochemical properties of proteinase.
关键词
蛋白酶 /
分光光度法
Key words
Penicillium marneffei /
Proteinase /
Spectrophotometric method
李民1 ;杨连娟1 ;章伟1 ; 章强强2.
马内菲青霉蛋白酶的体外诱导及其活性测定[J]. 微生物与感染. 2008, 4(4): 224-225
LI Min; YANG Lian-juan; ZHANG Wei; ZHANG Qiang-qiang.
In vitro induction of proteinase production of Penicillium marneffei and measurement of its activity[J]. Journal of Microbes and Infections. 2008, 4(4): 224-225
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