微生物与感染
2025年1月8日 星期三   首页 |  主编心语 |  期刊介绍 |  编委阵容 |  投稿指南 |  出版道德 |  期刊订阅 |  联系我们 | English
微生物与感染
  论著 本期目录 | 过刊浏览 | 高级检索 |
上海地区散发布氏杆菌感染的细菌学及分子鉴定
孟成艳; 金嘉琳; 阮斐怡; 雷建强; 陈澍; 张文宏

复旦大学附属华山医院感染科,上海 200040

Bacteriological and molecular identification of a sporadic case of Brucella infection in Shanghai
MENG Cheng-yan; JIN Jia-ling; RUAN Fei-yi; LEI Jian-qiang; CHEN Shu; ZHANG Wen-hong
Huashan Hospital; Fudan University; Shanghai 200040; China
全文: PDF (429 KB)   HTML (0)
输出: BibTeX | EndNote (RIS)      
摘要: 

目的 本研究对我院的1例散发布氏杆菌病患者进行细菌学及分子生物学的分析,并在国内首次尝试了用数目可变串联重复单元(VNTR)分子指纹分析法对其进行了基因分型并和国际流行株进行了分子流行病学比较分析。方法 对临床疑似布氏杆菌病病例作血液细菌培养与生化鉴定,进一步作布氏杆菌特异性基因片段的序列分析鉴定以及利用布氏杆菌基因组中的8个位点构建VNTR指纹图谱,参照国际布氏杆菌VNTR数据库,构建布氏杆菌基因系统树。结果 用细菌学方法确定散发疑似布氏杆菌病病例体内分离到的为布氏杆菌,通过基因序列分析进一步得到证实,但不能鉴定到生物种和生物型。对分离株作VNTR指纹分析提示该散发布氏杆菌病为猪2型布氏杆菌感染所致。结论 通过传统细菌培养方法与布氏杆菌VNTR指纹分析可用于我国布氏杆菌病分子流行病学的系统调查。

服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
孟成艳
金嘉琳
阮斐怡
雷建强
陈澍
张文宏
关键词 分子鉴定 猪2型布氏杆菌    
Abstract

Objective To identify a sporadic case of brucellosis found in Shanghai by bacteriological and molecular biological methods. Methods Blood culture combined with automatic biochemical identification system was used to isolate the Brucella strain from a patient suspected of brucellosis and a Brucella's specific secretary gene was amplified from the strain and analyzed by sequencing. We firstly multiple loci VNTR (Variable Number of Tandem Repeats) was used to analyze the Brucella genotype, by which Brucella dendrogram tree was established based on VNTR fingerprints according to the international published VNTR genotypes data. Results The isolated strain from this sporadic case was identified by bacteriological and molecular biological method as a kind of Brucella species. Although biochemical analysis and special gene sequencing could not identify the biovar of Brucella species, VNTR dendrogram of clustered VNTR genotypes indicated the case of brucellosis belonged to Brucella suis biovar 2.Conclusion Conventional bacterial cultures with biochemical and Brucella VNTR analysis can be used to identify the infection of Brucella and it is important investigate the molecular epidemiological profile of strains isolated in our country.

Key wordsBrucella    Molecular identification    Brucellasuis biovar 2
通讯作者: 张文宏   
引用本文:   
孟成艳; 金嘉琳; 阮斐怡; 雷建强; 陈澍; 张文宏. 上海地区散发布氏杆菌感染的细菌学及分子鉴定[J]. 微生物与感染 , 2006, 1(1): 32-34 .
MENG Cheng-yan; JIN Jia-ling; RUAN Fei-yi; LEI Jian-qiang; CHEN Shu; ZHANG Wen-hong. Bacteriological and molecular identification of a sporadic case of Brucella infection in Shanghai. Journal of Microbes and Infections, 2006, 1(1): 32-34 .
链接本文:  
http://jmi.fudan.edu.cn/CN/      或     http://jmi.fudan.edu.cn/CN/Y2006/V1/I1/32

版权所有 © 2008 《微生物与感染》编辑部
地址:上海市医学院路138号 邮编:200032
Tel:021-54237633 Fax:021-64434224    E-mail:jmi@fudan.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn